以“粤甜13号”单倍体种子为材料, 利用不同浓度秋水仙素直接浸泡单倍体种子, 同时设置加倍时间及加倍温度两个因素, 并对单倍体浸种方法进行优化。结果表明, 秋水仙素浓度、加倍时间和加倍温度均会影响甜玉米单倍体加倍效果, 通过秋水仙素直接浸泡甜玉米单倍体种子, 可显著提高甜玉米单倍体加倍率。如不考虑各因素间互作, 最适加倍浓度、处理时间及加倍温度分别为0.02%、30 h、32℃;若考虑各因素间互作, 0.02%浓度, 24℃处理32 h可获得较高的单倍体结实率, 药害率低, 且均优于自然加倍和常规秋水仙素处理。

In order to further improve the doubling efficiency of sweet corn haploid,“Yuetian13”haploid seeds as materials, different concentration colchicine was directly used to soak sweet haploid seeds, at the same time, doubling time and temperature were studied, and optimized the soaking seed method. The results showed that the colchicine concentration, doubling time and temperature effect the sweet corn haploid doubling ratio, soaked sweet corn haploid seeds directly by colchicine, can significantly improve the doubling ratio of sweet corn haploid. If not considering the interactions among various factors, the optimal doubling concentration, treatment time and temperature was 0.02%, 30 hours, 32℃ respectively, if considering the interactions among various factors, 0.02% concentration, 24℃ and 32 hours treatment could obtain more(doubled haploid, DH) line, but the Phytotoxicity ratio was very low, and was superior to the natural and normal colchicine doubling treatment, the research can provide reference for soaking seed doubling of sweet corn haploid.

广州市珠江科技新星项目(2013J2200083)、2014年院长基金项目[粤农科科技字(2013)14号]、广东省教育部产学研结合项目(2012B091100467)、广州市农业招标项目(GZCQC1202FG04002)、广东省旱地作物品种改良技术研究团队项目(No.2011A020102010)、2013年度广东省农业科学院作物所所长基金项目、广东-东盟科技合作平台建设(20110501)