以玉米品种先玉335和良玉99经高温高湿老化的种子为试材, 通过外源一氧化氮(NO)供体硝普钠(SNP)的不同浓度溶液浸种处理并进行发芽试验, 研究老化玉米种子的发芽、活力特性及萌发后幼苗的生理反应。结果表明, 老化玉米种子在一定的SNP浓度时, 两个品种均表现为生活力、发芽势、发芽指数、活力指数显著高于对照(P<0.05), 其中当SNP浓度为1.50和1.25 mmol/L 时以上各指标达最高;当SNP浓度为1.25 mmol/L时, 老化玉米种子萌发后幼苗的SOD、POD和CAT等活性酶含量最高(P<0.05), 其次是1.00 mmol/L处理。经SNP处理的老化种子提高了幼苗内的游离脯氨酸含量和可溶性糖含量, 但对MDA含量没有显著影响(P<0.05)。综合而言, SNP浓度为1.25 mmol/L时, 老化玉米种子的萌发能力、活力及幼苗生理特性改善和提高效果最显著。

The seeds from maize varieties Xianyu335 and Liangyu99 aged with high temperature and high humidity selected as experimental materials, through soaked in different concentrations of SNP of exogenous Nitric Oxide donor and then germinated. The germination character, vigor character and physiology response of germinated seedlings about the aging seeds were studied. The results showed that, under the action of some SNP concentration, the viability, germination energy, germination percentage and vigor index of the two varieties were higher than the check, especially 1.50 mmol/L and 1.25 mmol/L(P<0.05). The contents of SOD, POD and CAT were the highest at the SNP concentration 1.25 mmol/L, the next was 1.00 mmol/L, and the content of proline and soluble sugar from the seedlings of the aging seeds were higher than the check but the content of MDA showed no significant influence (P≤0.05). Therefore the germination ability, vigor and physiology character were significantly improved and enhanced under the SNP concentration 1.25 mmol/L.

国家自然科学基金青年基金项目(31200231), 吉林省教育厅项目(200953)、吉林省科技厅自然基金项目(201215185)