通过平板稀释法对来自青岛不同地区的海洋样品进行分离, 共得到18株海洋放线菌。采用浸虫法测定海洋放线菌发酵液对玉米螟2龄幼虫的室内毒力, 筛选得到2株活性较高的菌株YC3和SLR3, 其48 h发酵液对玉米螟2龄幼虫的校正死亡率分别为86.21%和81.04%。根据形态特征、培养特征及生理生化性状将活性菌株鉴定为链霉菌属(Streptomyces)。

Eighteen strain marine actinomyces were isolated from sea samples of Qingdao different regions by using dilution-plate method. Toxicities of fermentation broth from these marine actinomyces were assayed against 2 instar larvae of Asian corn borer by insect immersion bioassay method, and two strains had insecticidal activities for Ostrinia furnacalis G. The corrected mortality rates were 86.21% and 81.04% respectively in 48 h after treatment with supernatant from fermentation broth of YC3 and SLR3. The morphological and cultural characteristics, physiological and biochemical properties showed two strains were Streptomyces.

国家级大学生创新创业训练计划(201210435002)、“十二五”农村领域国家科技计划课题(BAD19B04-09)、山东省高等学校青年骨干教师国内访问学者项目、山东省“泰山学者”建设工程专项、青岛农业大学第六届大学生创新教育立项经费