以单交种先玉335作母本, 与京科诱006单倍体诱导系杂交, 产生单倍体及秋水仙素加倍形成双单倍体(DH)群体, 用SNP芯片对DH群体进行基因型分析, 获得1 337个在先玉335的双亲间有多态的SNP数据, 对SNP进行卡方检测(P<0.05), 发现516个SNP表现偏分离, 占38.6%, 这些偏分离SNP中有285个偏向母本PH6WC, 占55.2%;231个偏向父本PH4CV, 占44.8%。在玉米的8条染色体上, 发现10个偏分离热点区域(SDR), 分析SNP的χ²值与其在染色体上的位置之间的关系发现, χ²值最大的SNP往往在SDR的中心位置。

Haploids originating from the hybrid, Xianyu335 (PH6WC×PH4CV) were crossed with the haploid in-ducer, Jingkeyou006 and the resulting doubled haploid lines (DHs) were obtained by chromosome doubling with col-chicine. A total of 1337 SNP markers detected polymorphism between PH6WC and PH4CV. Among 1 337 SNP, cov-ering whole maize 10 chromosomes, five hundred and sixteen SNP (38.6%) showed the genetic distortion (P<0.05). Of the total segregation distortion SNP, two hundred and eighty-five SNP (55.2%) deviated toward female parent, PH6WC, while 231 SNP (44.8%) deviated toward male parent, PH4CV. Totally, tensegregation distortion regions (SDRs) were detected among 8 different chromosomes, the relationship between χ² valueand position in chromosome of SNP was analyzed.

北京市科技计划课题(D131100000213002)、国家科技支撑项目(2011BAD35B09)、北京市农林科学院科技创新能力建设专项(KJCX20140202)、“863”计划“玉米产量功能基因研究与克隆”(2012AA10A305)