利用玉米自交系齐319与郑58杂交衍生的重组自交系群体,通过筛选剩余杂合体(RHL)以达到快速构建CSSLs的目的。在接近纯合的重组自交系群体中,结合基因组重测序信息扫描玉米第5染色体上的InDel位点,定点开发分子标记,筛选剩余杂合体进行自交快速获得了多个位点的染色体片段代换系。在玉米5号染色体的19 130 718 bp～214 379 898 bp区间内,共获得41个染色体片段代换系。两个染色体片段代换系位点间的间距为0.2～26.4 Mb,平均为2.5 Mb。其中8个染色体片段代换系群体中只含有1个多态位点,为单片段代换系。本研究所建立的CSSLs可为玉米第5染色体上QTL的精细定位及功能分析提供支撑,并为玉米育种提供中间材料。

By a method of discovering residual heterozygous line(RHL) in the recombinant, inbred lines and CSSLs were produced after self-pollination. Based on re-sequenced data of maize genome through next generation sequencing, InDel markers were developed throughout chromosome 5 and 41 CSSLs were selected at average space of 2.5 Mb from the 19 130 718 bp to 214 379 898 bp. The minimal space between adjacent CSSLs was 0.2 Mb while the max was 26.4 Mb. Eight single segment substitution lines were produced containing only one polymorphic site. These CSSLs will be benefit for fine-mapping of QTLs located on chromosome 5 and also can achieve the develop-ment of plant molecular breeding from utilizing single gene to synthetically exploiting genome.

江苏省自然科学基金(BK20141385)、江苏省农业科技自主创新资金[CX(13)3060]、上海市能源作物育种及应用重点实验室开放课题