对细胞质雄性不育系DT-合344、ZT-合344和保持系合344雄穗不同发育时期小花中活性氧代谢相关指标进行比较。结果表明,雄穗整个生长阶段,不育系雄穗中超氧化物阴离子(O2·-)、丙二醛(MDA)含量和过氧化物酶(POD)活性高于保持系。随着雄穗的发育,保持系中超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、抗坏血酸过氧化物酶(APX)活性和可溶性蛋白含量逐渐高于不育系,达显著水平。雄穗小孢子孕育后,不育系小花中过氧化氢(H2O2)、脯氨酸(pro)、可溶性糖含量显著低于保持系。活性氧代谢的异常与玉米细胞质雄性不育有关。

Related indicators with activated oxygen metabolism were compared in florets tassel growth stages of cytoplasmic male steriler DT-he344, ZT-he344 and maintainer he344. The results showed that, in the tassel whole growth stage, superoxide anion(O2·-), malondialdehyde(MDA) content and peroxidase(POD) activity were higher in steriler tassel than in maintainer. With tassel development, superoxide dismutase(SOD), catalase(CAT), ascorbate peroxidase(APX) activity and soluble protein content of the maintainer were higher gradually than steriler and reached significantly. After tassel small spores breed, significantly lower hydrogen peroxide(H2O2), proline(pro), solu-ble sugar content were found in the steriler florets compared with those in the maintainer. Above showed abnormity of activated oxygen metabolism be associated with cytoplasmic male sterility in maize.

农业部公益性项目“本地与引进种质资源高效结合与利用研究”(201303007)、黑龙江省八一农垦大学博士启动基金(XYB2013-03)、黑龙江省高校寒地作物品种改良与生理生态重点开放实验室项目(ZWXQDJ-6)