The yield and quality of maize were influenced by maize prolificacy, which is regulated by many factors such as environment and genotype. As an important plant growth regulator, auxin plays an important role in organ formation and differentiation. The transgenic plants with DR5rev::mRFPer and pZmPIN1a::ZmPIN1a::YFP were used to analyze the distribution pattern of the auxin response signal labeled by red fluorescent protein and the auxin transport protein PIN1a labeled by yellow fluorescent protein, respectively. It was found that the axillary bud of different nodes was at the same stage of development in the early stage of plant development. Auxin response signal and auxin transport protein are evenly distributed throughout the axillary bud of primordia. With the growth of the plant, the development of axillary buds at different nodes began to be out of sync. The patterns of auxin distribution and poplar auxin transport were significantly different between axillary buds at different nodes, and the upper axillary bud showed stronger auxin-responsive signals and polar auxin transport signals. These results suggest that auxin local synthesis and polar transport play a role in the development of axillary buds of maize.