在玉米基因组水平鉴定13个rboh蛋白基因，分别定位于6条染色体上。家族蛋白被分成3个亚组，第2亚组成员ZmrbohB、ZmrbohD和ZmrbohG基因均含有14个外显子、定位叶绿体；其余两个亚组成员具有不同亚细胞定位，其中第1亚组基因间具有较强的正向选择压力。Zmrbohs基因在不同组织和发育阶段的表达模式不同，其中ZmrbohD、ZmrbohE和ZmrbohM为组成型高表达，ZmrbohC和ZmrbohL在花药特异表达。通过转录测序数据分析，ZmrbohB、ZmrbohD、ZmrbohE、ZmrbohG、ZmrbohI、ZmrbohK和ZmrbohM等7个基因不同程度参与对低温、高温、盐、紫外和干旱胁迫的应答。

The respiratory burst oxidase homolog(rboh) is the main pathway for generating reactive oxygen species(ROS) in plants. It plays a vital role in plant growth and development and stress response. In this study, 13 rboh protein genes were identified at the genome of maize using bioinformatics analysis, located on 6 different chromosomes. The family proteins may be divided into three subgroups, and the members of the second subgroup, ZmrbohB, ZmrbohD, and ZmrbohG, contained 14 exons and chloroplast localization. The members of the other two subgroups have different subcellular localization, among which the first subgroup has intense positive selection pressure. The expression patterns of the Zmrbohs gene were different in various tissues and development stages, in which ZmrbohD, ZmrbohE, and ZmrbohM constitutively highly expressed, and ZmrbohC and ZmrbohL specifically expressed in anthers. Through transcription-sequencing data analysis, seven genes, including ZmrbohB, ZmrbohD, ZmrbohE, ZmrbohG, ZmrbohI, ZmrbohK, and ZmrbohM, were involved in response to low temperature, high temperature, salt, UV-B, and drought stress to varying degrees.

黑龙江省自然基金重点项目（ZD2020C007）、黑龙江八一农垦大学青年创新人才项目（CXRC2016-02）