以200份玉米优良自交系为材料，2022-2023年夏分别进行小斑病菌的田间接种，综合两年数据共鉴定出高抗小斑病的玉米自交系3份，分别为K22、CML50和齐319；筛选到包括Mo17在内的抗性玉米自交系24份。选取K22和B73两个具有不同抗性水平的玉米自交系进行小斑病菌的室内接种。结果表明，48 h和72 h时，自交系K22叶片的病斑数、病斑面积和病斑面积比等各项指标均显著优于自交系B73，进一步验证田间鉴定的准确性。48 h或72 h时，高抗小斑病自交系K22与感病对照B73相比，5种防御酶活性增加14.32%～70.45%。

Two hundred excellent inbred lines of maize were used as materials, and the field inoculation of SLB pathogen were conducted during the summers of 2022 and 2023, respectively. Three highly resistant inbred lines, namely K22, CML50, and Qi319, were identified and 24 resistant inbred lines, including Mo17, were screened based on the two-year data. Additionally, two maize inbred lines with different levels of resistance, K22 and B73, were selected for indoor inoculations with the SLB pathogen and the results showed that the various indexes of leaf lesion number, leaf lesion area and leaf lesion area ratio for K22 were significantly better than those for B73 at 48 and 72 hours, which furtherly validated the accuracy of field identification. The activity increase of 5 defense enzymes ranged from 14.32 to 70.45% in the highly resistant inbred line K22 compared to the susceptible control B73 while 48 or 72 hours.

S435.131

国家自然科学基金(32061143033)、河南省科技攻关项目(232102110255)、河南省农业科学院自主创新项目(2024ZC015)、2024年河南省留学人员科研择优项目