利用优良玉米自交系郑58和B73杂交构建的F_2:3家系为材料，对其经过单倍体诱导系诱导获得的单倍体植株在多环境下对株高进行表型鉴定及遗传研究。结果表明，使用完备区间作图法(ICIM)共定位到8个与株高相关的QTL位点，分别位于1、4、5、7和10号染色体，定位结果解释表型变异介于3.98%～21.04%。其中,qPH4-2在多个环境下被定位到，表型贡献率(Phenotypic variation expland，PVE)介于14%～21%，为主效QTL位点；另一主效位点qPH4-1所在的bin4.08区间包含候选基因GRMZM2G393337，可使细胞伸长受到抑制而导致矮化。对F_2:3家系单倍体株高使用RRBLUP模型进行GS，5倍交叉验证平均预测精度为0.50。随着训练群体大小和标记数目的增加，预测精度会先增加随后趋于平缓。当训练群体占总体的60%或当SNP标记数目达到300时，即可得到较高的预测精度。

Haploids derived from the F_2:3 families of the maize elite inbred lines Zheng 58 and B73 were used for QTL mapping and GS in this study. A total of 8 QTLs related to plant height were mapped on chromosomes 1, 4, 5, 7 and 10 by inclusive composite interval mapping(ICIM). The phenotypic variation explained(PVE) value of each QTL ranged from 3.98% to 21.04%. The QTL qPH4-2 was mapped in multiple environments with PVE value ranging from 14% to 21%, which was a major QTL. Another major QTL qPH4-1 located at bin 4.08 contains the candidate gene GRMZM2G393337, which can inhibit cell elongation and lead to dwarfing. RRBLUP model was used for GS of haploids plant height of the F_2:3 families, and the average prediction accuracy of five-fold cross validation was 0.50. When the training population size and marker density increase, the prediction accuracy would first increase rapidly then tend to be flat. When 60% of the total genotypes were used as the training population or 300 SNP markers were used, a relative high prediction accuracy can be achieved.

S513.035.3

自治区天山创新团队项目(2022D14017)、自治区自然基金重点项目(2022D01D034)、国家自然科学基金项目(U2003304,32060484,32001561)、新疆主要作物生物育种创新工程项目(2021A02001-2,2021B02002-2-2)