采用盐碱土壤盆栽方法, 以T7代转BcWRKY1 基因玉米株系WL-1、受体自交系LH1037、常规自交系K10为试材, 分析其在不同生长时期对土壤酶活性和土壤理化性质的影响。结果表明, 在播种后20、40、60、80 d对根际土壤进行检测, 转BcWRKY1 基因玉米与受体亲本自交系和普通玉米自交系对照相比, 土壤碱性磷酸酶的活性不存在差异显著性, 个别取样点对土壤蔗糖酶、脱氢酶和脲酶活性存在显著差异;土壤电导率均无显著差异, 在个别取样时期对土壤pH值和SAR值存在显著差异。这些显著性差异只在个别取样时期短暂存在, 没有持续出现, 且受体自交系与普通自交系不同品种间也具有显著差异, 很难确定说明这些差异是由转基因玉米本身造成。

A transgenic salt-tolerant maize line with BcWRKY1 gene in T7-generation was used to analyze the effects on rhizosphere saline soil enzyme activities and physicochemical properties in different growth stages by pot experiment. The results showed that there were no significantly differences on soil alkaline phosphatase activity among transgenic line, acceptor line and common control inbred line at four sampling time. The significantly differences were detected in soil sucrase, dehydrogenase and urease activities at individual sampling time. There was no significant impact of transgenic line on soil conductivity at all four sampling time. The significant differences were obtained in pH value and sodium adsorption value at individual sampling time. The above significant differences appeared at some sampling time briefly and there was no long-time effect on the soil. Due to significant differences between receptor and common inbred line, the effects were not caused by transgene.

黑龙江省留学归国人员科学基金(LC201411)、转基因重大专项“ 转基因玉米小麦大豆环境安全评价技术”(2014ZX08011003)