选用西北灌区生产上具有代表性的10个玉米品种，采用不同接种方法及接种浓度在玉米不同生育时期人工接种玉蜀黍黑粉菌，研究抗性鉴定技术，筛选出一整套重复性好、发病完全、受外界因素干扰小的接种技术体系。结果表明，供试玉米品种接种玉蜀黍黑粉菌的最佳方法是注射法，最佳接种时期为6叶1心期，最佳接种浓度为9×10³～10×10³个孢子/mL。

Effect on incidence rate of common smut on 10 different resistant varieties of corn in northwest of China, in field conditions by different inoculating methods, inoculating time, dosages for inoculation were compared. The results indicated that the most effective inoculating method was to inject spores into the corn plant, which caused the highest incidence rate of Ustilago maydis. The optimal time of inoculation was at 6 leaf-stage of corn plant. The optimal dosage of the pathogen for injection was at 9×10³ - 10×10³ spore/mL.

国家科技支撑计划课题“高淀粉活秆成熟玉米筛选及栽培技术集成示范”专题(2007BAD52B08)